Journal: Journal of Cancer
Article Title: Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor
doi: 10.7150/jca.25279
Figure Lengend Snippet: The efficacy of MPHOSPH1 knockdown using small-interfering RNA (siRNA). A . Protein was extracted from the cell lines at 24 h and 48 h post-transfection. Knockdown of MPHOSPH1 was confirmed by Western blotting ( A ). MPHOSPH1 knockdown significantly reduced cell migration ( B ) and cell invasion ( C ) in both NEC8 and NEC14 ( p <0.001 for both). MPHOSPH1 knockdown also significantly inhibited cell proliferation ( D ) and colony formation ( E ) in both of the cell lines ( p <0.001 for both).
Article Snippet: The human embryonal carcinoma cell lines NEC8 and NEC14 were purchased from the Riken Bioresource Center (Tsukuba, Japan) and cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS).
Techniques: Knockdown, Small Interfering RNA, Transfection, Western Blot, Migration